How does manual annotation work?
Clones from the ‘clone mapping and sequencing’ approach are subject to manual annotation to find all genes in them. For this, the alignments of ESTs, cDNAs or protein sequences (supporting evidence) to the genomic region are investigated and a gene is added if the match is of sufficient quality, continuous and features correct splice sites. Genes are not built on ‘guesses’ like the results of gene prediction programs. Once a clone is manually annotated, it is submitted to EMBL/Genbank and can be downloaded from there and/or browsed in the Vega database.
Clones from the ‘clone mapping and sequencing’ approach are subject to manual annotation to find all genes in them. For this, the alignments of ESTs, cDNAs or protein sequences (supporting evidence) to the genomic region are investigated and a gene is added if the match is of sufficient quality, continuous and features correct splice sites. Genes are not built on the results of ab initio gene prediction programs. Once a clone is manually annotated, it is submitted to EMBL/Genbank and can be downloaded from there and/or browsed in the Vega database.
