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How does one calculate the purity and quantity of a DNA sample?

April 26, 2017CALCULATE dna purity quantity sample

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How does one calculate the purity and quantity of a DNA sample?

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Quantity and quality of DNA is accomplished by measuring absorbance at A260 (DNA), A280 (protein) and A320 (background). For DNA quantitation: A260 A320 = corrected DNA quantity. 1 absorbance unit at A260 = 50 ng/ml (be sure to correct for any dilutions) For DNA purity (protein carry over) measure A260-A320 / A280-A320.