How does palmitate specifically sequester Ca2+ pools utilizing this GTP-mediated prefusion Ca2+ transfer?
2. This palmitate/GTP induced Ca2+ increase was correspondent to increasing concentrations of palmitate ( below critical micelle formation concentration). 3. This GTP dependent increase was specific to differing fatty acid chain lengths C12-C20. C15 and C16 (palmitate) showed clearly substantial increases as compared to other fatty acids which showed little Ca2+ accumulation. Similarly, unsaturated fatty acids were unable to induce an increase in Ca2+. 4. The action of palmitate/GTP was similar to the action of oxalate/GTP suggesting a similar mechanism. Both oxalate and palmitate induced accumulation was reversed upon the addition of palmitoyl-CoA, as expected from previous experiments. How do palmitate and oxalate enter the pools? 5. An anion transport-inhibitor DIDS was able to block and prevent the palmitate/oxalate induced Ca2+ accumulation. Are there differences between the states of these differentially sequestered pools? 6. IP3 induced a significantly greater decrease of Ca2+ f
