How is Microsatellite genotyping done?
Genotyping using microsatellite markers involves PCR amplification of the microsatellite region and measurement of the size of the amplified fragment. For a genome-wide scan we use fluorescently labelled primers for the PCR that amplify 400 microsatellites spread across the human genome. These markers are spread at an average genetic distance of 10 cM. We also have a set of primers that amplify markers spread at 5 cM.
