How long are the cDNAs? Is the submitted sequence the entire sequence of the cDNA?
We have devoted considerable effort to generate high quality libraries in which (with the exception of the CK library) a significant percentage of clones are full length. To provide a rough estimate of the degree to which we succeeded in synthesizing full length clones, we examined the start sites of 100 of our LD cDNAs, each of which corresponds to a different gene where previous researchers had deposited a putatively full-length cDNA sequence in GenBank. In 75 out of 100 cases our cDNA clone was within 100 bases of the longest cDNA in GenBank for that gene. In 29 out of 100 cases our cDNA was longer than the longest cDNA in GenBank. We have reanalyzed those clones that are represented in the DGC and we estimate that 80% of clones extend beyond the 5′ start site while 34.5% of clones are longer than the the corresponding clone in the Genbank test set. (See table 1 of Science paper.
Related Questions
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- I submitted a cDNA sequence but notice it appears in the database as an mRNA sequence, why is this?
- How long are the cDNAs? Is the submitted sequence the entire sequence of the cDNA?
