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I am working on a multi-span transmembrane protein. Should I boil my sample before SDS-PAGE fractionation?

April 26, 2017boil Fractionation multi-span protein sample sds-page transmembrane working

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I am working on a multi-span transmembrane protein. Should I boil my sample before SDS-PAGE fractionation?

1 Answer

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No. According to our experience, the boiling of transmembrane protein in SDS sample buffer tends to induce protein aggregation. We suggest that the customer incubate the over-expression lysates with 1xSDS sample buffer at room temperature for 30 minutes before loading.