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I don get the sharp peak by using H2O as an eluent. What is the problem?

April 26, 2017eluent h2o peak problem Sharp

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I don get the sharp peak by using H2O as an eluent. What is the problem?

1 Answer

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Change the eluent from H2O to buffer. The characteristics of the target molecules may be influenced by such variables as pH. A sharper peak can be obtained by using suitable buffer adjusted for target molecules.