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In Round 1 of RampUP, why is only half of the T7/T3-modified cDNA used in the IVT reaction?

April 26, 2017cDNA Half ivt rampup REACTION round t7 Used

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In Round 1 of RampUP, why is only half of the T7/T3-modified cDNA used in the IVT reaction?

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Using half of the T7/T3-modified cDNA in the IVT reaction helps to keep non-specific amplification products low, while allowing high yield amplification after two rounds. It also eliminates the need to purify the cDNA prior to the first IVT reaction.