In the SpermMar IgG test the latex particles agglutinate, is this normal?
Yes it is. Just as in the principle explained above, the antiserum not only links the latex particles to the spermatozoa but also to the other latex particles which also contain IgG. The particles therefore tend to agglutinate. Another reason why agglutination may occur is because the bottles containing the latex particles was not mixed well before use. Also make sure to thoroughly mix the antiserum with the latex particles and the sperm on the slide.