Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Interval and BED format Extract Genomic DNA tool under Fetch sequences section returns one less nucleotide for each of my intervals. Is this a bug?

April 26, 2017bed dna Extract fetch format genomic interval section sequences Tool

0

Posted

Interval and BED format Extract Genomic DNA tool under Fetch sequences section returns one less nucleotide for each of my intervals. Is this a bug?

1 Answer

0

Posted

Genomic intervals (Interval and BED formats) on Galaxy are 0-based, start-inclusive, and end-exclusive, in accordance with the definition of BED format by UCSC. As a result, a BED interval defined as “chr1 100 200” will cover nucleotides from 100 to 199. For a detailed explanation, please read this article.