Is it possible to double stain my gel with silver stain and a coomassie dye stain?
Yes. Our researchers have stained with coomassie dye stains, both before and after staining with the Color Silver Stain. (Counter-intuitively, silver stains detect heavily loaded proteins poorly, and double-staining with coomassie dye remedies this problem). When double staining, avoid dark background by halving the silver staining incubation time or by diluting the silver solution. Consider using GelCode Blue Stain Reagent (Product # 24590 or 24592).
