Is it possible to first transform the BJ5183 with pAdenoVator E1/E3 and then make this recombinant strain electrocompetent for transformation with the linearized transfer vector?
A. No, it is not possible. Plasmids grown in BJ5183 are very susceptible to rearrangements. Growth and amplification of plasmids should not be performed in that strain of bacteria. That is why the DH5 strain is also included in the AdenoVatorTM kit, it allows for amplification without rearrangements (does not support recombination).
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