My biomolecule has a molecular weight greater than 150 kDa. Will the conjugation kit work for me?
Although the conjugation will still likely occur, the purification of molecular weights greater than 150 kDa using our eFluor® Nanocrystal Kits is unlikely. You can still use the kit(s), but you will have to purify the conjugate from un-reacted biomolecules using a technique capable of resolving the two molecular weights. Effective techniques for separation include size-exclusion chromatography, ion-exchange chromatography, or hydrophobic interaction chromatography.
