My experiment requires me to run 48 gels but we only have access to one Typhoon Variable Mode Imager. How can I ensure that all my gels are scanned within 24 hours of running?
We recommend that gels are run overnight in batches of 12 (i.e. one Ettan DALT run per day). Gels can then be scanned the following day when the electrophoretic separation has finished. An experiment of this size would require staggered gel runs over four days, followed by scanning on the day each run is completed. For IEF, all 48 strips can be focused simultaneously and the strips stored at –15 oC to –30 oC until required for 2-D electrophoresis.
Related Questions
- My experiment requires me to run 48 gels but we only have access to one Typhoon Variable Mode Imager. How can I ensure that all my gels are scanned within 24 hours of running?
- The volume supplied for the FBC is insufficient to run the sample in closed mode. Can the QAP provide larger volume for the FBC sample?
- How do I clean the Typhoon Variable Mode Imager platen and sample press?
