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Should the combination of Buffer B with the sonicated cells be cloudy or clear before proceeding to the immunoprecipitation?

April 26, 2017buffer cells clear cloudy combination immunoprecipitation proceeding sonicated

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Should the combination of Buffer B with the sonicated cells be cloudy or clear before proceeding to the immunoprecipitation?

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The sheared chromatin must be clear before proceeding to the immunoprecipitation. Thus, you can add Buffer A or rub the tube between your hands to make the sample clear.