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Sometimes when I run the Coamatic FVIII assay, I see an upward drift in my activity from the first to the last samples. Why is this?

April 26, 2017activity assay coamatic drift fviii run samples upward

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Sometimes when I run the Coamatic FVIII assay, I see an upward drift in my activity from the first to the last samples. Why is this?

1 Answer

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Thrombin, 1 NIH unit, is included in the factor reagent. It has been shown that thrombin can activate FX, and over time you will see an increase of up to 5% activity. If you are doing numerous samples at once on an automated instrument, you might want to consider Coatest FVIII.