What amounts of DNA and primer are required for the sequencing reactions?
For each sequencing reaction, customers should send 1 µg of template DNA and at least 10 pmoles of primer in a final volume of 10 µl. If you are sequencing with a universal primer or if Vimta has your sequencing primer, the tube should only contain 1 µg of template in a total volume of 9 µl. We will add 1 µl (containing 10 pmol) of primer.