What are the advantages to use plasmid-based RNAi expression vectors?
Plasmid-based RNAi expression vectors have several advantages over the synthetic siRNA duplexes: First of all, shRNAs/miRNAs expressed from a plasmid vector work better and last longer than synthetic siRNA duplexes. Secondly, DNA vectors are stable and easy to handle. Transfection with plasmid DNA on eukaryotic cells is a familiar technique in many research labs. Moreover, a vector carrying selection marker allows you to establish stable cell lines to observe the long-term effects of gene silencing. Finally, a plasmid-based RNAi expression vector is cost-effective. Preparation of an RNAi expression vector construct is a one-time investment. Unlike synthetic siRNA duplexes, which have to be repeatedly ordered, plasmid DNA can be easily maintained and amplified at low costs. Through innovation, LBL scientists have added special features (see below) onto plasmid-based RNAi expression vectors, which allow you to perform RNAi experiments far beyond the capacity of what synthetic siRNA duple
