What are the benefits to Top Reading versus Bottom Reading on my fluorescence microplate reader?
Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA or protein quantification. Bottom reading should provide better results when working with adherent cells, on assays such as GFP gene expression. If in doubt, you can run both Top and Bottom measurements in the same protocol and compare the results to make your final choice.
Related Questions
- Why are the fluorescence filters on the Modulus II Microplate Multimode Reader individual fluorescence optical kits, not tunable monochromator or filter wheels or filter slides?
- Why are the fluorescence filters on the Modulus Microplate Multimode Reader individual fluorescence optical kits, not tunable monochromator or filter wheels or filter slides?
- What are the benefits to Top Reading versus Bottom Reading on my fluorescence microplate reader?
