What are the column buffers recommended for chitin chromatography and on-column cleavage?
A column buffer of 20 mM Tris-HCl, pH 8.5 containing 500 mM NaCl is recommended for the IMPACT purification step. Cleavage buffer should be freshly prepared using the column buffer supplemented with 50 mM 2-mercaptoethanesulfonic acid at 4°C. Other possible buffer conditions: 10-100 mM Tris-HCl, pH 8.0-9.0, 100-2000 mM NaCl.
