What are the reasons for poor, low, or inconsistent DNA yields on a NanoDrop after immunoprecipitation?
• Using the Nanodrop Diagenode has found that at low DNA concentration (less then 10 ng/µl) the values are not correct. The measurement at these concentrations is at the limit of detection and it results in inaccurate and inconsistent values. • Note that DNA purified with the slurry still contains peptides resulting from proteinase K digestion, causing inaccurate NanoDrop readings. • Keep in mind that the DNA is mostly in single-stranded form after boiling, so choose the corresponding option when taking the OD on the Nanodrop.
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