What are the recommended protocols for antigen retrieval?
Antigen retrieval is a very passionate subject among those who do immunohistochemistry. The tape sections are quite hardy and withstand antigen retrieval well. Although we do not recommend using a microwave for antigen retrieval with the array slides, it can be done. The TARP Lab uses a rice cooker/vegetable steamer or pressur cooker. Other methods that work include the use of autoclave ovens. The choice of buffer and antigen retrieval time is antigen dependent. We have not noticed appreciable spot loss except under extreme conditions.
