What are the typical conditions used for the high density growth media?
High density growth media can be used in 14 ml tubes or in any flasks with or without baffles. Both glass and plastic containers may be used. these growth media are designed to grow bacterial cells at 370C. Temperatures lower than 370C may be used for protein expression. Typical shaking speeds for high density growth media in a baffled flask is 350 rpm and 450 rpm in a regular (non-baffled) flask. All containers should be balanced and securely fixed in a shaker incubator. In addition, the incubator and incubation room must be sufficiently ventilated for cultures of 500 ml or more. High density growth media may be used the same as common media except that they need better aeration and will support higher cell density.
