What buffer is recommended?
The more stringent the buffer used the better (i.e., higher concentrations of salt and detergent in the buffer will lead to cleaner results). It is essential that the buffer is optimized for every new chipping experiment as a compromise must be found between low backgrounds and detrimental effects on the target. NP40 can be used as a detergent and RIPA is also commonly used for X-ChIP.
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