What constitutes an experiment?
The TPA database was created as a repository for annotations that are derived as a result of wet-bench experiments based on existing nucleotide sequence deposited in the DDBJ/EMBL/GenBank databases. Supporting evidence for valid TPA sequences includes using the nucleotide sequence information to study expression of the RNA in various tissues and to study promoter elements. Conceptual translations have been used for a variety of protein studies, including GST-fusion analyses and enzyme kinetics. These are just some examples of the studies that can be done. Computational studies on their own do not constitute experimental evidence and must be accompanied by biological experiments that support the new annotation.
