What did you do with the lpr and gld (generalized lymphoproliferative disease) mice?
I thought that if we could get lpr mice, it would be easy to examine whether lpr was a mutation of CD95. The lpr mice were not commercially available at that time in Japan. I searched Medline for Japanese scientists working with lpr mice, and found a group in Tokyo that had the mice. In July 1991, I went to Tokyo and obtained several mice from Dr. Katagiri at the Institute of Medical Science, University of Tokyo. I returned with the mice to Osaka via Super Express (the charming young lady who had the next seat in the train had an unpleasant 3-h trip due to the bad smell of the mice). In Osaka, Ms. Rie Watanabe-Fukunaga prepared RNA from the lpr mice, and found that the CD95 mRNA was not expressed in their thymus or spleen! She then prepared DNA from the mice and found a rearrangement in the CD95 gene. There is also a different allele for lpr called lprcg. I once again went to Tokyo and obtained lprcg mice from Dr. Matsumura. The mice were transported to Osaka with me this time by airpl
I thought that if we could get lpr mice, it would be easy to examine whether lpr was a mutation of CD95. The lpr mice were not commercially available at that time in Japan. I searched Medline for Japanese scientists working with lpr mice, and found a group in Tokyo that had the mice. In July 1991, I went to Tokyo and obtained several mice from Dr. Katagiri at the Institute of Medical Science, University of Tokyo. I returned with the mice to Osaka via Super Express (the charming young lady who had the next seat in the train had an unpleasant 3-h trip due to the bad smell of the mice). In Osaka, Ms. Rie Watanabe-Fukunaga prepared RNA from the lpr mice, and found that the CD95 mRNA was not expressed in their thymus or spleen! She then prepared DNA from the mice and found a rearrangement in the CD95 gene. There is also a different allele for lpr called lprcg. I once again went to Tokyo and obtained lprcg mice from Dr. Matsumura. The mice were transported to Osaka with me this time by airpl
