What does the Lentiviral packaging vector contain (pFIV-PACK)? Do I need the pFIV-PACK packaging vector?
The packaging vector provides all the proteins essential for transcription and packaging of an RNA copy of the expression construct into recombinant viral particles. To produce a high titer of viral particles, transiently co-transfect packaging cells (HEK 293T cells) with the expression and packaging vectors.