What is melting curve analysis?
The melting point of double-stranded PCR products can be used to determine the size of the DNA fragments amplified, (eliminating the need for agarose gels). Melting curve protocols are performed with standard PCR reagents but require a fluorescent dye that binds double stranded DNA (typically SYBR green), and use of a special thermocycler (see Resources for non-standard genotyping section). Melting curve analysis protocols provided on JAX® Mice datasheets can be used as a starting point for standard PCR, by eliminating the SYBR green and optimizing the protocol for use in a standard thermocycler.
