What is the basic procedure for using the MultiSep®216 together with the MycoSep® 225 Trich or 227 Trich+ columns?
Essentially, the MultiSep®213 and MultiSep®216 columns are used when additional clean up is necessary (complex matrixes – finished feed, etc.) In this case, we recommend the use of a MycoSep® column followed by additional clean up of the purified extract in a MultiSep® column. Similar to the MycoSep®227 Trich+ or MycoSep®225 Trich column, the charcoal alumina packing in the MultiSep®213 or 216 column will bind the interferences. Because of this absorption, minimal toxins retained must be rinsed off the columns using extraction solvent. MultiSep®216 column require only 12ml of rinse while the MultiSep®213 column requires 25ml of rinse. The described procedure can be used for trichothecene clean up only.
