What is the best RNA purification method to use to get total RNA for the TargetAmp Kit?
Total cellular RNA, isolated by a number of methods, including salt-fractionation (for example EPICENTRE’s ArrayPure™ and MasterPure™ RNA purification kits), TRIzol® / TRI Reagent and spin columns, can be amplified successfully using the TargetAmp RNA Amplification Kits. However, it is very important that the purified RNA be free of salts, metal ions, ethanol and phenol which can inhibit the enzymatic reactions performed in the RNA amplification process. Details of total RNA isolation methods are presented in the TargetAmp Kit product literature that accompanies each kit and is available on this web site.
