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What is the best way to resolve topoisomers in an agarose gel system?

agarose best gel resolve system
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What is the best way to resolve topoisomers in an agarose gel system?

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• Cast a 1-1.5% gel in 0.2ug/ml chloroquine in TPE buffer (36 mM Tris-Cl, pH7.8, 30mM NaH2PO4, 1 mM EDTA). • Run gel in TPE buffer at 15 volts for 15 hrs. at room temperature. • Stain with 0.5ug/ml Ehtidium bromide • Destain well (15-30 min) with H2O and photograph.

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