What is the difference between siRNA and diced siRNA (d-siRNA)?
The structure of the molecules is the same dicer specifically cleaves long dsRNA into the 21-23 nucleotide duplexes with a two-nucleotide overhang that is the hallmark of siRNA. A key difference is that d-siRNA typically contains a pool of siRNA generated from the entire length of a long dsRNA target, whereas siRNA generally refers to a single sequence that is specific to a particular target region, and is often synthesized as a single oligo or a specified combination of several oligos.
