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What is the difference between using hepatocytes and microsomes?

April 26, 2017difference hepatocytes microsomes

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What is the difference between using hepatocytes and microsomes?

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10 Posted

Primary fresh or cryopreserved hepatocytes contain a more complete metabolic system, including Phase I and Phase II metabolism, and are a stronger predictor of in vivo metabolic stability. Liver microsomes contain a significant portion of Phase I enzymes; therefore, significant disappearance of the test compound in the presence of liver microsomes likely indicates contributions from CYP450 enzymes. Liver microsomes require additional components in order to examine Phase II metabolism.