What is the general workflow?
In the afternoon of the first day, the arrays are blocked for one hour to prevent non specific binding of accompanying proteins (background). The samples, containing 30 µg nuclear protein, are applied to the arrays and incubated over night at room temperature in the thermoblock for slides on a thermomixer comfort / R. The next day, the detection and staining of the slides is performed. Detection happens by antibodies, specific for the activated status of the TFs. Staining can be done by fluorescence or the Silverquant® system. The Silverquant method was developed by Eppendorf. It offers a 10 times enhanced sensitivity and perfect photo stability compared to fluorescence.
