What is the highest temperature that AMV Reverse Transcriptase can be used in?
For RT-PCR, AMV Reverse Transcriptase can be used up to 60 °C. For amplicons expected to be 1 kb or less even 65 °C can be used. Although AMV RT is rapidly inactivated at this temperature it remains active for the period of time that is necessary for transcription of short products. For PCR products expected to be greater than 1 kb a maximum first strand synthesis temperature of 60 °C is suggested. Be sure your first-strand primer anneals at the high temperature.
