What quality control steps are taken to ensure high quality sequencing results?
Sample: We use a combination of Bioanalyzer, spectrophotometer, and gel electrophoresis to determine initial quality of the samples received from the customer and to check the progress of the library preparation at various steps. Library: We use a combination of gel electrophoresis and Qbit fluorometer to ensure the library is at an acceptable fragment size and concentration. Sequencing: We check the number of clusters generated per tile for the first cycle of sequencing to make sure that it is within an acceptable density level to be sure chemistry is functioning properly. The total number of raw reads is also an indication of run performance and quality. Additionally, one of the sequencing lanes may be reserved for a quality control sample. The data from this lane must meet certain quality criteria in order for the run to be deemed successful. Data: There is a standard Illumina base calling and data filtering program that is applied to the raw data to remove low quality reads. Additi
