What running buffer can be used with Elchrom gels?
A19: All Gels are run in standard TAE buffer. The final concentration of the running buffer should be 30 mM TAE (according to Maniatis this equals a 0.75 x solution). Elchrom offers a standardised TAE buffer stock solution (P/N 3031). One packaging unit consists of 20 x 50 ml tubes with 40 x TAE buffer (1.2M Tris-Acetate- EDTANa). Borate buffer (TBE: Tris Borate EDTA Buffer) must NEVER BE USED, AS BORATE WILL DESTROY THE GELS! Even smaller traces of borate buffer will influence the property of the gel already.
