What should I do if my negative controls used in the immunoprecipitation (IgG and only beads not treated with any antibody) are positive for c-fos in my qPCR?
It looks like there is a problem with cross-contamination. • Make sure the stock solution of beads is not contaminated. • Run a negative control for the PCR (sample without template DNA) to assure the contamination did not occur with the PCR reagents. • If you work with strip of tubes, pay attention to caps – always put them in “right” order and remove them carefully not to cross-contaminate the samples. • Check to see if you get the same results using alternative primers pairs.
Related Questions
- Can the efficiency of the immunoprecipitation (testing the positive and negative controls) be assessed using endpoint PCR instead of qPCR?
- top of page 21. Do external positive and negative controls need to be run with each new kit used?
- Do you supply positive or negative controls for the SpermMar IgG test ?
