What specific media components are usedin the Overnight Express Autoinduction Systems?
The Overnight Express Autoinduction Systems use an optimized blend of carbon sources, including lactose. The carbon sources are metabolized differentially to initially promote tightly regulated, uninduced growth, followed by autoinduction with lactose and continued cell growth, which results in high cell density at harvest. OnEx™ Solution 1 provides the blend of carbon sources. To grow bacterial cultures to high cell densities, metabolic acids must be buffered to maintain pH near neutral. For increased protein expression, additional nitrogen is also necessary. OnEx Solution 2 provides both a concentrated buffer and an additional nitrogen source, so that cultures attain high cell densities and increased protein expression, even when grown in shake flasks or 96-well plates. Magnesium is essential for cell growth, and higher concentrations of magnesium have been shown to increase the viability of cultures stored at 4˚C for several weeks (1). OnEx Solution 3 provides the critical magnesium
