What steps can I take to get best possible enrichment from methylated DNA immunoprecipitation?
• Use the correct concentration of antibody as recommended in the manual. • Double check the amount of DNA and antibody • Use the recommended ratio of antibody to DNA – the ratio is critical. • Always use fresh dilutions of antibody (within the same day). • Avoid excessive freeze-thaw of the antibody – you may aliquot the antibody upon receipt, freeze the aliquots, and prepare fresh dilutions before each use. • Assure that the purification of the immunoprecipitated DNA is as recommended in the manual. Always remember to add Buffer B (from Table 5 in the manual) to achieve good immunoprecipitation.
