When running the assays, are they analyzed using allelotyping or is there a separate application for analyzing quantitative genotyping or allele specific expression?
• Relative quantification assays are run with TYPER Analyzer using Allelotyping. Absolute quantification assays for gene copy number or allele specific expression, etc. should be run with the QGE Analyzer so that the standard curves can be viewed and the EC50 of each allele can be calculated by the software. You also mentioned that you’d use double stranded competitor for analyzing DNA. Synthesizing a long oligo is expensive and I know that a number of customers would like to avoid doing this. Isn’t possible just to divide you result by a factor of 2? • The double-stranded competitor acts to ensure that the wild-type template and competitor are amplified with the same kinetics during competitive PCR. Users can make the competitor double-stranded via PCR if desired and then purify with a column. Actually determining a bias in competitive PCR is a difficult task since PCR is exponential the templates are competing for the same primers. Does the QGE software calculate the absolute quantit
