Which protocol should I use to enrich for miRNA?
If you are enriching for LMW RNA using the PureLink™ miRNA Isolation Kit, we recommend enriching from total RNA as opposed to cells or tissue. The PureLink™ miRNA Isolation Kit manual includes a protocol for large sample amounts that starts with the isolation of total RNA using TRIzol® Reagent. We recommend using this protocol in NCode™ labeling applications, even if you are not starting with large sample amounts.