Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Why can’t I cleave out the insert when digesting with enzymes that were used for cloning the construct?

April 26, 2017cleave cloning construct digesting enzymes insert Used

0

Posted

Why can’t I cleave out the insert when digesting with enzymes that were used for cloning the construct?

1 Answer

0

Posted

A restriction site used for cloning can be disrupted if different restriction enzymes causing identical overhangs at the end of vector and insert were applied. In this case, you have to choose the next suitable polylinker restriction sites flanking the insert.