Why didn’t I see the 28S and 18S rRNA bands in the gel?
The 28S and 18S rRNA bands may migrate with the genomic DNA in a native 0.8% agarose gel. However, if you add some salts (e.g., 30 mM NaOAc, pH unadjusted) to the loading dye, you should get a good separation of the 5S, 18S, 28S rRNA, and the genomic DNA bands. Alternatively you can do a DNase I digestion to remove the DNA before running the gel.
