Why do false negatives or false positives ocurr?
Many factors can contribute to both false negatives and false positives results. False Negatives poor assay sensitivity, assay not broadly reactive to detect all known strains, inhibition of PCR reaction that is not monitored by an internal control, testing samples from animals within the diagnostic gap with assays showing inferior sensitivity, overpooling of samples, human error, improper instrument performance, deviations from approved assay protocols, etc. False Positives lack of assay specificity, cross contamination, human error, etc.
