Why do I need to transfer the IgY Microbeads into a fresh spin column after 25 cycles?
While the IgY-Microbead resin is durable for 100 cycles under proper conditions, the spin column frit flow rates sometimes drop after 30 uses. For this reason, we recommend transferring the 50% slurry with a 1 mL disposable pipette tip into a fresh spin column after 25 cycles. By repeating this transfer three times (after cycles 25, 50 & 75), the spin column resin can be used 100 times. We feel that this simple mechanical transfer is more reliable than including chaotropic agents, such as urea, in the stripping buffer.
