Why do you not have a rhodamine phosphoramidite or CPG?
RESPONSE:The tetramethyl rhodamine (TAMRA) molecule seems to be insufficiently stable to ammonium hydroxide to reliable survive the conditions of deprotection. It can be added to the appropriate location in an amino modified oligonucleotide using the NHS ester and the conditions described in the User Guide on Page 75. We have subsequently found that TAMRA is stable to the conditions of UltraMild deprotection, so TAMRA-CPG (29-5910) and TAMRA-dT (10-1057) are now both available. REFERENCE(S): Glen Research User Guide to Modification and Labelling, 1999, 75.
