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Why the size limit of 200 base pairs?

base limit pairs size
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Why the size limit of 200 base pairs?

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There is a size limit for TSA for two reasons. First, with the increased size of sequence generated by the new sequencing technologies, for the sequence to be an assembly it would be expected to be larger than the smallest DNA generated by these machines. Second, to receive acceptable coding region prediction results a minimum of 200 base pairs is required.

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