Will the RNAi Facility have RNAi reagents available for species other than human?
The RNAi Facility currently maintains and distributes only the Hannon/Elledge human retroviral shRNA library. However, we will continue to assess needs and investigate the possibility of adding other RNAi collections, including those targeting other species (for example, mouse) or in other delivery systems (for example, lentivirus). Additionally, we have negotiated a Duke-wide ‘Custom siRNA/shRNA Vector Construction Service’ agreement through an external vendor to provide shRNA synthesis, cloning into expression vector, and sequence verification (see Question 17 below). Return to top.
