Will the Typhoon system do chemiluminescence?
A. Yes. For high-sensitivity DNA and protein detection on blots, Molecular Dynamics recommends chemifluorescence. Benefits include direct quantitation, no film exposures, and increasing signal strength for up to 12 hours. Chemifluorescent samples can be scanned up to two weeks following sample preparation with no significant loss in signal or resolution. Chemifluorescent reagent kits for Southern, northern and western blotting are available from Amersham Pharmacia Biotech. For more information and a thorough comparison of chemiluminescence and chemifluorescence, see Molecular Dynamics’ publication “Understanding Chemifluorescence.
A. Yes. For high-sensitivity DNA and protein detection on blots, Molecular Dynamics recommends chemifluorescence. Benefits include direct quantitation, no film exposures, and increasing signal strength for up to 12 hours. Chemifluorescent samples can be scanned up to two weeks following sample preparation with no significant loss in signal or resolution. Chemifluorescent reagent kits for Southern, northern and western blotting are available from Amersham Pharmacia Biotech.
